Hydroperoxide formation during autoxidation of conjugated linoleic acid methyl ester

Rauvolfia serpentina, commonly known as Sarpgandha, is a plant well-known for being utilized for several medicinal purposes. Unrestricted collection from natural stands and overexploitation for medicinal and traditional purposes have rendered it endangered, hence there is necessary requirement for the development of cultivation protocols for mass propagation and sustained utilization of the plant. In the present study, in vitro culture of the apical parts of R. serpentina on MS (Murashige and Skoog) medium enriched with NAA resulted in development of callus, whereas multiple shoot regeneration along with callus development was achieved on medium combination MS + NAA + Kn and MS + NAA + BAP. MS + 4µM NAA + 4µM BAP was found to be most optimum media composition for regeneration of shoots and callus. Among different media combinations utilized for in vitro rooting, a maximum of 82.6 % explants developed in vitro roots on ½ MS + 12µM NAA. Gas chromatography-mass spectrometry (GC-MS) analysis of methanolic extract prepared from leaves of wild and micropropagated plants of R. serpentina revealed the presence of 38 and 48 phytocompounds, respectively. 9, 12-Octadecadienoic acid, Methyl linoleate, Methyl stearate, Hexadecanoic acid, methyl ester, Linoleic acid, Ergost-4, 7, and 22-trien-3.alpha.-ol were some of the major compounds found to be present in the leaves of wild plants, and Linoleic acid, methyl ester, Cis-Linoleic Acid Methyl Ester, Methyl elaidate, hexadecanoic acid, and methyl ester were major compounds found to be present in in vitro raised plants. Many of the compounds detected have been known to possess 1 or more biological or pharmacological activities.

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Influence of Oryzanols Concentrate on the Oxidation of Methyl Ester Linoleic Acid and Study of Their Oxidation Products

Journal of Food Research ◽

10.5539/jfr.v8n4p37 ◽

2019 ◽

Vol 8 (4) ◽

pp. 37

Author(s):

Bruno Irigaray ◽

Iván Jachmanián ◽

María A. Grompone

Keyword(s):

Linoleic Acid ◽

Methyl Ester ◽

Sunflower Oil ◽

Rice Bran Oil ◽

Methyl Esters ◽

Acid Methyl Ester ◽

Oxidation Products ◽

Antioxidant Power ◽

Linoleic Acid Methyl Ester ◽

Acid Methyl

Oryzanols are frequently found in rice bran oil but almost completely removed in the neutralization step when the oil is chemically refined. In this way, oryzanols can be recovered from the soapstocks to generate a concentrate. Thereby, they could be used as antioxidants in lipids for specific purposes. In the present work the antioxidant power of oryzanols concentrate (33% purity) was studied together with pure oryzanols and butylhydroxytoulene (BHT). Methyl esters were prepared from regular sunflower oil without antioxidants to which the antioxidants before mentioned were added in an effective concentration of 3x10-3 M. The samples were oxidized in a heating block with oxygen flow and the hydroperoxides of linoleic acid methyl ester were analyzed. It was observed that all antioxidants were able to protect the sunflower oil methyl esters from oxidation with respect to methyl esters without antioxidants. Oryzanols presented a notoriously lower protection ability compared to BHT. However, the formation of the linoleic acid methyl ester hydroperoxides and their proportion, for the same oxidation stage, did not show differences between the antioxidants used. Therefore, the oxidation kinetics were similar between the different antioxidants studied.

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Selective production ofcis-9,trans-11 isomer of conjugated linoleic acid fromtrans-vaccenic acid methyl ester byDelacroixia coronata

Journal of Applied Microbiology ◽

10.1111/j.1365-2672.2008.04139.x ◽

2009 ◽

Vol 106 (5) ◽

pp. 1697-1704 ◽

Cited By ~ 9

Author(s):

A. Ando ◽

J. Ogawa ◽

S. Sugimoto ◽

S. Kishino ◽

E. Sakuradani ◽

...

Keyword(s):

Linoleic Acid ◽

Methyl Ester ◽

Conjugated Linoleic Acid ◽

Vaccenic Acid ◽

Acid Methyl Ester ◽

Acid Methyl

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Reduction of Detrimental Effect of Soybean Oil in-vivo Using Watermelon White Rind Extract

Asian Food Science Journal ◽

10.9734/afsj/2019/v7i429979 ◽

2019 ◽

pp. 1-6

Author(s):

Hanan M. El-Ghandour

Keyword(s):

Fatty Acid ◽

Linoleic Acid ◽

Methyl Ester ◽

Soybean Oil ◽

Aqueous Extract ◽

Calcium Level ◽

Acid Methyl Ester ◽

Negative Control ◽

Acid Methyl

Aim: To study the effect of white rind extract on decreasing soybean oil impact on calcium and phosphorous blood levels in vivo. Method: Dried watermelon white rind was directed to mycotoxin and elemental determinations to assure its safe usage. Soybean oil was subjected to fatty acid and GC-MS analysis. Biological experiment was conducted using male albino rats fed diet prepared by soybean oil and supplied with aqueous watermelon white rind extract for two months’ interval period. At the end of the experiment, the calcium and phosphorus in blood were determined. Results: The rind was free from aflatoxin and ochratoxin. Watermelon white rind aqueous extract contained iron, copper, potassium, chromium and selenium at concentration ranges of 3.4, 0.53, 45.51, 0.0142 and 0.0985 ppm, respectively. Soybean oil had free fatty acid, peroxide value, iodine number and anisidine value of 0.43%, 13.62 meq O2/Kg, 132 and 0.7, respectively. GC-MS analysis of soy oil ascertained the presence of twenty-four compounds: linoleic acid, methyl ester (25.27%), monensin (15.75%), elaidic acid (9.24%), nonadecanoic acid, methyl ester (7.04%), cis-13-eicosenoic acid (4.92%), cis-vaccenic acid (4.68%), linoleic acid (4.67%), palmitoleic acid (4.46%), 9-tetradecenal (4.42%) and cysteine (4.18%)were the most predominant. Fatty acid profile of the oil showed that the ratio of saturated fatty acid to unsaturated fatty acids was 1:5. Conclusion: Rats fed diet prepared by soybean oil had a decreased calcium level in comparison with negative control (p<0.05). Supplementation with watermelon white rind aqueous extract rendered calcium level to normal status as negative control. Phosphorus level wasn’t affected by soya oil.

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N-Acetylborrelidin B: a new bioactive metabolite from Streptomyces mutabilis sp. MII

Zeitschrift für Naturforschung C ◽

10.1515/znc-2017-0140 ◽

2018 ◽

Vol 73 (1-2) ◽

pp. 49-57 ◽

Cited By ~ 5

Author(s):

Abdelaaty Hamed ◽

Ahmed S. Abdel-Razek ◽

Marcel Frese ◽

Daniel Wibberg ◽

Atef F. El-Haddad ◽

...

Keyword(s):

Linoleic Acid ◽

Methyl Ester ◽

Bioactive Compounds ◽

Screening Program ◽

Acid Methyl Ester ◽

In Vitro Cytotoxicity ◽

Cervix Carcinoma ◽

Nmr Studies ◽

Acid Methyl

AbstractIn the course of our screening program for new bioactive compounds, a naturally new 18-membered macrolide antibiotic,N-acetylborrelidin B (1) along with borrelidin (2) were obtained from the marineStreptomyces mutabilissp. MII. The strain was isolated from a sediment sample collected in the Red Sea at the Hurghada Coast and characterized taxonomically. Additional nine diverse bioactive compounds were reported: 6-prenyl-indole-3-acetonitrile (3), sitosteryl-3β-d-glucoside, campesterol, ferulic acid, linoleic acid methyl ester, linoleic acid,N-acetylanthranilic acid, indole 3-acetic acid methyl ester, indole 3-carboxylic acid, and adenosine. Structure1was confirmed by in-depth NMR studies and by mass spectra, and comparison with related literature data. The antimicrobial activity of the strain extract and compounds1and2were studied using a panel of pathogenic microorganisms. The in vitro cytotoxicity of compounds1and2as well as the crude extract were tested against the human cervix carcinoma cell line (KB-3-1).

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Studying the Physicochemical Properties and Isolation of Unsaturated Fatty Acids from Edible Oils by GC-MS and Argentated Silica Gel Chromatography

Iraqi Journal of Science ◽

10.24996/ijs.2021.62.2.1 ◽

2021 ◽

pp. 346-362

Author(s):

Salim Najmaldain Saber ◽

Hikmat Ali Mohamad ◽

Madzlan Aziz

Keyword(s):

Fatty Acids ◽

Oleic Acid ◽

Fatty Acid ◽

Linoleic Acid ◽

Methyl Ester ◽

Physicochemical Properties ◽

Olive Oil ◽

Unsaturated Fatty Acids ◽

Acid Methyl Ester ◽

Acid Methyl

The core objective of this study was to investigate the physicochemical characteristics and fatty acid composition of the oils of sunflower, olive, virgin coconut and ginger oils, as well as the separation of their unsaturated fatty acids. The data indicated a significant variation in physicochemical properties (acid, saponification, ester, and iodine values) among oils. Transesterification process was carried out at a molar ratio of 1:7:0.1 of oil: methanol: KOH. Fatty acid methyl esters of oils were analyzed by infrared (IR) and gas chromatography–mass (GC-MS) spectrometry. Twelve fatty acids were identified, where the major fatty acid of olive oil was found to be oleic acid (89%), whereas those of sunflower and ginger oils were linoleic acid (80.9 %) and (79.3 %), respectively. Sunflower and olive oils were fractionated by 25% silver nitrate-impregnated silica gel column chromatography. By this method, linoleic acid methyl ester from sunflower and oleic acid methyl ester from olive oil were isolated with high purity percentages and yields. This study is significant for the development of food and pharmaceutical products.

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